Testkits are designed for culture, identification and enumeration of urogenital mycoplasma species: Mycoplasma hominis (M.h.) and Ureaplasma urealyticum (U.u.), using a liquid growth medium. The technique gives convincing advantages compared to agar cultivation:
- Test performance in a selective medium (µl-quantity) with higher sensitivity
- Incubation at 37°C; no CO2 supply necessary
- Results are given within 24 hours, in some cases after 48 hours
- Uniform reading of results by colour change of growth medium (yellow to red)
- Long shelf life of testkits: about 9 months. Storage temperature 4-8°C.
The principle of mycoplasma detection and identification is based on specific metabolic properties and natural antibiotic resistance of mycoplasma species:
Ureaplasma (U.u.): Hydrolysis of urea and resistance to lincomycin
Mycoplasma (M.h.): Hydrolysis of arginine and resistance to erythromycin
Metabolism causes liberation of ammonia resulting in a colour change of the medium which contains phenol red indicator. Samples for the testing of mycoplasma: Urethral, endocervical and vaginal swabs, sperm, gastric secretions.
Kits are available for a different range of testing:
- Identification (= I) and Enumeration (= Titer = T)
- Antimicrobial susceptibility testing (= A)
The antimicrobial susceptibility testing is provided for 9 antibiotics (A9).
The A9 testing includes resistance against: Lincomycin, Erythromycin, Roxythromycin, Azithromycin, Josamycin, Minocycline, Doxycycline, Ofloxacin, Ciprofloxacin.
||Article / Description
||Range of Tests
||Number of Tests
||Myco View® Screening-40
||Screening (optional I + T + A9)
||MycoView® ID 20
||I + T (optional A9)
||MycoView® RT 20
||I + T + A9
Testing mycoplasma with the MycoView® Screening-40 is done in a vial of growth medium and is designed as a qualitative assay. A positive result needs to be confirmed by a test assay that includes the identification of the type of mycoplasma. We recommend further testing using the MycoView® A9-trays (nor included in the kit, article of the kit MycoView® RT 20).
MycoView® ID 20
Testing mycoplasma with the MycoView® ID 20 is done in strips composed of 5 wells which includes a growth control and the identification of high and low mycoplasma titer. A determination of resistance may follow using MycoView® A9-trays (not included in the kit, article of the kit MycoView® RT 20).
MycoView® RT 20
Testing mycoplasma with the MycoView® RT 20 (ZDRT20) is done in strips composed of 12 wells which include a growth control, the identification and the antibiotic resistance testing against 9 antibiotics (A9).
All MycoView® kits need refrigeration at 2-8°C. They are products of ZEAKON Diagnostics (France).