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Cultivation Procedures

Serology®

The kit Serology® is designed for the use of serum and enables the quantitative detection (titration) of antibodies against the urogenital mycoplasma species Mycoplasma hominis (M.h.) and Ureaplasma urealyticum (U.u.). The principle of the test is a metabolic inhibition technique of mycoplasma test strains growing in the presence of a patient serum containing antibodies against mycoplasma species.  Shelf life is up to 12 months.

Reference Article / Desciption Number of Tests
IMMS14 Serology-14 14


The Serology® testkit needs refrigeration at 2-8°C. Is a product of ELITech (France).

 

Candida

Reference Article / Description Number of Tests
IMCA30 Candifast®-30    30

The Candifast® kit allows the identification of the main human pathogenic yeasts and the determination of resistance to 7 antifungal agents. In a preliminary procedure, a pure colony needs to be isolated from a solid agar plate. Following suspension in mediums, the cultivation of the yeast is done in a test tray containing 2 rows with 8 wells each (µl quantities). One row stays for the identification the other row is used for the resistance testing. The identification of the yeast is based on

  • the fermentation of various sugars
  • the susceptibility of strains to actidione
  • the demonstration of urease activity

Interpretation help is given by a coloured chart included in the kit. The determination of resistance is noted by the inhibition of growth in the presence of 7 antifungal agents which are:


Amphotericin B, Nystatine, Flucytosine, Econazole, Ketoconazole, Miconazole, Fluconazole.


Growth and metabolism of the yeast is visualized by a colour change of the medium either to yellow or to fuchsia. Incubation temperature is 37°C; reading of the result within 24-48 hours.


Shelf life of kits may vary between 6-8 months.  The Candifast® testkit needs refrigeration at 2-8°C. It is a product of ELITech (France).

 

MYCOPLASMA

Testkits are designed for culture, identification and enumeration of urogenital mycoplasma species:  Mycoplasma hominis (M.h.) and Ureaplasma urealyticum (U.u.), using a liquid growth medium. The technique gives convincing advantages compared to agar cultivation:

  • Test performance in a selective medium (µl-quantity) with higher sensitivity
  • Incubation at 37°C; no CO2 supply necessary
  • Results are given within 24 hours, in some cases after 48 hours
  • Uniform reading of results by colour change of growth medium (yellow to red)
  • Long shelf life of testkits: about 9 months. Storage temperature 4-8°C.

The principle of mycoplasma detection and identification is based on specific metabolic properties and natural antibiotic resistance of mycoplasma species:

Ureaplasma (U.u.): Hydrolysis of urea and resistance to lincomycin
Mycoplasma (M.h.): Hydrolysis of arginine and resistance to erythromycin

Metabolism causes liberation of ammonia resulting in a colour change of the medium which contains phenol red indicator. Samples for the testing of mycoplasma: Urethral, endocervical and vaginal swabs, sperm, gastric secretions.

Kits are available for a different range of testing:

  • Identification (= I) and Enumeration (= Titer = T)
  • Antimicrobial susceptibility testing (= A)

The antimicrobial susceptibility testing is provided for 9 antibiotics (A9).
The A9 testing includes resistance against: Lincomycin, Erythromycin, Roxythromycin, Azithromycin, Josamycin, Minocycline, Doxycycline, Ofloxacin, Ciprofloxacin.

Reference Article / Description Range of Tests Number of Tests
ZDMS40 Myco View® Screening-40 Screening (optional I + T + A9)    40
ZDID20 MycoView® ID 20 I + T (optional A9)    20
ZDRT20 MycoView® RT 20 I + T + A9    20


MycoView® Screening-40

Testing mycoplasma with the  MycoView® Screening-40 is done in a vial of growth medium and is designed as a qualitative assay. A positive result needs to be confirmed by a test assay that includes the identification of the type of mycoplasma. We recommend further testing using the MycoView® A9-trays (nor included in the kit, article of the kit MycoView® RT 20).

MycoView® ID 20

Testing mycoplasma with the MycoView® ID 20 is done in strips composed of 5 wells which includes a growth control and the identification of high and low mycoplasma titer. A determination of resistance may follow using MycoView® A9-trays (not included in the kit, article of the kit MycoView® RT 20).

MycoView® RT 20

Testing mycoplasma with the MycoView® RT 20 (ZDRT20) is done in strips composed of 12 wells which include a growth control, the identification and the antibiotic resistance testing against 9 antibiotics (A9).


All MycoView® kits need refrigeration at 2-8°C. They are products of ZEAKON Diagnostics (France).